This technical overview highlights the sensitivity and resolution of the Agilent Fragment Analyzer and TapeStation systems to detect adapter dimers lower than the recommended threshold of 0.5%.
The automated electrophoresis instrument portfolio includes systems for objective assessment of sample integrity, concentration, and fragment size. Download this eBrochure for an interactive experience.
The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable quality control (QC) of nucleic acids for a broad variety of applications, including next-generation sequencing (NGS) libraries, RNA samples, PCR amplicons, and CRISPR workflows.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The HS Small Fragment kit is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The Small Fragment kit (p/n DNF-476-0500) is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next-Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The DNF-474 HS NGS Fragment assay is designed for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The NGS Fragment kit (1–6000 bp) (p/n DNF-473- 0500 and DNF-473-1000) is used for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.
小片段和 NGS 试剂盒
小片段试剂盒涵盖的分子量测定范围为 50–1500 bp，是小片段、cfDNA 和小 RNA 文库样品 QC 分析的理想试剂盒。NGS 片段试剂盒的分子量测定范围可以扩展到 6000 bp，专注于 NGS 文库 QC。小片段和 NGS 片段试剂盒专门针对高起始量浓度范围，而 HS 小片段和 HS NGS 片段试剂盒设计用于较低的浓度范围。
确定 NGS 文库中是否存在接头二聚体的质量控制步骤
随着测序平台的灵敏度不断提高，采取相应措施来确保测序文库中不良产物含量尽可能低至关重要，因为这些不良产物很可能影响测序结果的准确性。使用 HS NGS 或 HS 小片段试剂盒，安捷伦片段分析仪系统可以准确评估 NGS 文库中接头二聚体的百分比。这些信息可以帮助研究人员在其测序工作流程中做出关键决策。
使用 HS 小片段试剂盒分析游离 DNA
质量分析在确定提取的循环游离 DNA (cfDNA) 的分子量和纯度方面起着重要作用。片段分析仪系统利用 HS 小片段试剂盒可为前三个核小体峰提供出色的分辨率和分子量测定。该试剂盒可对包含或不包含载体 RNA 的提取 cfDNA 进行高效、出色的分离。
使用小片段和 NGS 试剂盒进行分子量测定的最佳实践
对于许多核酸应用来说，分子量测定的 QC 检查点必不可少，它可确保实验获得成功。片段分析仪系统与 NGS 片段、HS NGS 片段、小片段和 HS 小片段试剂盒相结合，可对 50 至 6000 bp 的片段和弥散条带进行可靠的分子量测定。
Detection of Adapter Dimers in NGS Libraries with the Agilent Fragment analyzer and TapeStation Systems
This technical overview highlights the sensitivity and resolution of the Agilent Fragment Analyzer and TapeStation systems to detect adapter dimers lower than the recommended threshold of 0.5%.
Build Consistency into Your Lab’s Workflow with the Agilent Automated Electrophoresis Portfolio.
The automated electrophoresis instrument portfolio includes systems for objective assessment of sample integrity, concentration, and fragment size. Download this eBrochure for an interactive experience.
Nucleic Acid Analysis for Sample Quality Assessment Using the Agilent Fragment Analyzer Systems
The Fragment Analyzer systems utilize automated parallel capillary electrophoresis to provide reliable quality control (QC) of nucleic acids for a broad variety of applications, including next-generation sequencing (NGS) libraries, RNA samples, PCR amplicons, and CRISPR workflows.
Agilent DNF-477 HS Small Fragment Kit Quick Guide for Fragment Analyzer Systems
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The HS Small Fragment kit is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.
Agilent DNF-476 Small Fragment Kit Quick Guide for Fragment Analyzer Systems
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The Small Fragment kit (p/n DNF-476-0500) is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next-Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.
Agilent DNF-474 HS NGS Fragment Kit Quick Guide for Fragment Analyzer Systems
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The DNF-474 HS NGS Fragment assay is designed for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.
Agilent DNF-473 NGS Fragment Kit (1-6000 bp) Quick Guide for Fragment Analyzer Systems
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The NGS Fragment kit (1–6000 bp) (p/n DNF-473- 0500 and DNF-473-1000) is used for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.